pGLO lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 0 none none - pGLO LB/amp 0 none none + pGLO LB/amp 6 none purplish white + pGLO LB/amp/ara 2 none greenish white

2.	What two new traits do your transformed bacteria have?
	Each got the plasmid and the bacteria they also create a colony and they were green when you flash the fluorescent light on it.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	We were spreading from one  bacteria to the petri dish.
4.	What is the role of arabinose in the plates?
	It got the plasmid to glow green that was protein plasmid that grew in  all of the colonies in the petri dish.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	One use is to use green fluorescent protein as an indicator to see if the organism got another desired trait. Another use for green fluorescent protein in animals as protection. Another use for green fluorescent protein is glow sticks.
6.	Give an example of another application of genetic engineering.

              Seed Companies genetically seeds so that they are resistant diseases and pesticides.