Recombinant DNA lab

In this lab, we learned about recombinant DNA. It would provide resistant to Kanamycin resistance for antibiotics would this plasmid provide resistance would be important. I think antibiotic resistance would be important because it help protect from harmful diseases. I didn't use Ava II and Hin dIII, Bam HI, Bgl II, Hpa II,  Sac I, Xma I and Asp II and LIGASE because it doesn't work because each different restriction enzyme. The 3 antibiotic were tetracycline, kanamycin, and ampicillin in each trial I had to be aware of whether these are resistant. The one we used was Eco RI because it was the closest.