Tuesday, October 6, 2015

Egg Macromolecule Lab conclusion

Egg Macromolecule Lab
In this lab we asked the question can macromolecules be identified in an egg cell. I found that in an egg cell, the egg membrane contains monosaccharides, polysaccharides, protein, and lipids. The egg yolk contains monosaccharides, polysaccharide, protein, and lipids. The egg white contains  polysaccharides and proteins. I found that different types of egg such as egg white, egg yolk, egg membrane had different types of macromolecules. When tested for monosaccharide, the egg membrane in the qualitative observation turned green/purple and the egg yolk, for the qualitative observations, turned greenish blue when treated with Benedict’s solution. When tested for polysaccharide in which the egg cell was treated with iodine, the egg membrane turned to brownish color, the egg yolk turned orange color and the egg white turned light orange. When tested for protein in which the egg cell was treated with NaOH and CuSO4, the egg membrane turned blue, the egg yolk turned green and egg white turned dark blue. When tested for lipids in which the egg is treated with Sudan III, the egg membrane turned tinted orange and the egg yolk turned yellow mixed with red.
Our data was unexpected because an error that affected the data which is that I didn’t observed the eggs carefully enough. This error could have affected my data by skewing the numbers for the quantitative amount of macromolecules. Due to these errors in future experiments I would recommend to make sure to observe more carefully and record the correct information. I would also recommend that all of the group members say their opinion on the color.
This lab was done to demonstrate the different types of macromolecules in the different parts of the egg cell. From this lab I learned about different types of test to look at which macromolecules are present. I also learned which macromolecule are present in different parts of the egg which helps me understand the concept of where the macromolecule are in the cell and cell biology. Based on my experience from this lab, I can apply the concepts with a cell. The procedure can be improved by using two eggs for each test to see if the color is consistent. Another situation that applies to look at your skins on your fingertips when in water.

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